DNA Polymerases
DNA polymerases are vital molecular biology enzymes that catalyze the synthesis of complementary DNA strands by assembling nucleotides from a DNA template. They are essential for applications like PCR (Polymerase Chain Reaction), DNA sequencing, and gene cloning. Key parameters include:
- Synthesis Direction: 5′ to 3′
- Key Mechanisms: Exonuclease activity (proofreading) and high fidelity
Product Description
Core Features & Specifications
- Processivity & Speed: Measures how long the enzyme stays attached to the DNA strand before detaching. High-processivity enzymes like Invitrogen Platinum SuperFi DNA Polymerase are optimized for long, difficult, or GC-rich amplicons.
- Fidelity: Determines the enzyme’s accuracy in reading the template and preventing errors (mutations). Standard enzymes like Taq have higher error rates, while high-fidelity variants reduce errors up to 50-fold.
- Thermostability: Essential for PCR. Thermostable polymerases (e.g., Phusion High-Fidelity DNA Polymerase sourced from Pyrococcus bacteria) can withstand repeated denaturation cycles up to 95°C without degrading.
