Reverse Transcriptase Enzymes
Reverse Transcriptase (RT) enzymes are RNA-dependent DNA polymerases used to synthesize complementary DNA (cDNA) from single-stranded RNA templates. They are fundamental reagents for molecular biology, enabling gene expression analysis, RT-PCR/RT-qPCR, RNA sequencing, and cDNA library construction.
Product Description
Core Product Features
- RNA to DNA Conversion: Catalyzes the transcription of single-stranded RNA into double-stranded DNA.
- Sequential Activities: Features RNA-dependent DNA polymerase, ribonuclease H (RNase H) for degrading the initial RNA template, and DNA-dependent DNA polymerase for second-strand synthesis.
- High-Yield Formatting: Engineered for high thermal stability, allowing reactions to occur at higher temperatures (50°C to 65°C) to break down complex RNA secondary structures.
- Reduced RNase H Activity: Many modern engineered enzymes are modified to lack RNase H activity, which helps preserve long RNA transcripts for full-length cDNA synthesis.
